Cadherin 5 anticorps (pTyr731)

N° du produit ABIN7218860

L’anticorps Lapin Polyclonal anti-Cadherin 5 a été validé pour WB, ELISA et IHC (p). Il convient pour détecter Cadherin 5 dans des échantillons de Humain et Souris.

Aperçu rapide pour Cadherin 5 anticorps (pTyr731) (ABIN7218860)

Antigène: Cadherin 5 (CDH5)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cadherin 5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Cadherin 5 anticorps

Épitope: pTyr731

Fonction: VE-Cadherin (phospho Tyr731) Polyclonal Antibody

Specificité: Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from human VE-Cadherin Phospho-Tyr731

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur Cadherin 5

Antigène: Cadherin 5 (CDH5)

Autre désignation: VE-Cadherin

Sujet: Rabbit Anti-VE-Cadherin (phospho Tyr731) Polyclonal Antibody,CDH5, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD antigen CD144,Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin-5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74 % homology.,Cadherin-5

Poids moléculaire: observerd band 130kDa

ID gène: 1003

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2