E2F2 anticorps (AA 190-270)

N° du produit ABIN7214646

L’anticorps anti-E2F2 Polyclonal Lapin est utilisé pour la détection de E2F2 dans des échantillons de Humain et Souris. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour E2F2 anticorps (AA 190-270) (ABIN7214646)

Antigène: E2F2 (E2F Transcription Factor 2 (E2F2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp E2F2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-E2F2 anticorps

Épitope: AA 190-270

Fonction: Rabbit Anti-E2F-2 Polyclonal Antibody

Specificité: E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur E2F2

Antigène: E2F2 (E2F Transcription Factor 2 (E2F2))

Autre désignation: E2F-2

Sujet: E2F2, Transcription factor E2F2, E2F-2E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.

Poids moléculaire: 48kD

ID gène: 1870

UniProt: Q14209

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication