EIF2A anticorps (pSer51)

N° du produit ABIN7203754

Cet anticorps anti-EIF2A Polyclonal Lapin (ABIN7203754) détecte spécifiquement EIF2A dans WB, IHC, IF et ELISA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Porc et Poisson.

Aperçu rapide pour EIF2A anticorps (pSer51) (ABIN7203754)

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivité: Humain, Souris, Rat, Chien, Porc, Poisson

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Détail du produit anti-EIF2A anticorps

Épitope: pSer51

Fonction: Rabbit Anti-eIF2α (phospho Ser51) Polyclonal Antibody

Specificité: Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human eIF2alpha Phospho-Ser51

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,Not yet tested in other applications,

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur EIF2A

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Autre désignation: eIF2alpha

Sujet: EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alphaThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).

Poids moléculaire: 38kD

ID gène: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly