LIM Domain Kinase 1 anticorps (pThr508)

N° du produit ABIN7213028

L’anticorps anti-LIM Domain Kinase 1 Polyclonal Lapin est utilisé pour la détection de LIM Domain Kinase 1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC et IF.

Aperçu rapide pour LIM Domain Kinase 1 anticorps (pThr508) (ABIN7213028)

Antigène: LIM Domain Kinase 1 (LIMK1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIM Domain Kinase 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-LIM Domain Kinase 1 anticorps

Épitope: pThr508

Fonction: Rabbit Anti-LIMK-1 (phospho Thr508) Polyclonal Antibody

Specificité: Phospho-LIMK-1 (T508) Polyclonal Antibody detects endogenous levels of LIMK-1 protein only when phosphorylated at T508.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human LIMK-1 Phospho-Thr508

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur LIM Domain Kinase 1

Antigène: LIM Domain Kinase 1 (LIMK1)

Autre désignation: LIMK-1

Sujet: LIMK1, LIMK, LIM domain kinase 1, LIMK-1There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. LIM domain kinase 1 also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Poids moléculaire: 72kD

ID gène: 3984

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events