PIAS4 anticorps (AA 420-500)

N° du produit ABIN7223058

Cet anticorps Lapin Polyclonal détecte spécifiquement PIAS4 dans WB, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour PIAS4 anticorps (AA 420-500) (ABIN7223058)

Antigène: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIAS4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PIAS4 anticorps

Épitope: AA 420-500

Fonction: PIASy Polyclonal Antibody

Specificité: PIASy Polyclonal Antibody detects endogenous levels of PIASy protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogène: Synthesized peptide derived from the C-terminal region of human PIASy at AA range: 420-500

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Commentaires:

Primary Antibody

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Détails sur PIAS4

Antigène: PIAS4 (Protein Inhibitor of Activated STAT, 4 (PIAS4))

Autre désignation: PIASy

Sujet: Rabbit Anti-PIASy Polyclonal Antibody,PIAS4, PIASG, E3 SUMO-protein ligase PIAS4, PIASy, Protein inhibitor of activated STAT protein 4, Protein inhibitor of activated STAT protein gamma, PIAS-gamma,The protein inhibitor of activated Stat (PIAS) proteins, which include PIAS1, PIAS3, PIASx, and PIASy, were originally characterized based on their interaction with the Stat family of transcription factors. PIAS1, PIAS3, and PIASx interact with and repress Stat1, Stat3, and Stat4, respectively. Deletion of PIAS1 leads to inhibition of interferon-inducible genes and increased protection against infection. The PIAS family contains a conserved RING domain that has been linked to a function as a small ubiquitin-related modifier (SUMO) ligase, coupling the SUMO conjugating enzyme Ubc9 with its substrate proteins. Numerous studies have now shown that PIAS family members can regulate the activity of transcription factors through distinct mechanisms, including NF-κB , c-Jun, p53 , Oct-4, and Smads. The activity of PIAS1 is regulated by both phosphorylation and arginine methylation. Inflammatory stimuli can induce IKK-mediated phosphorylation of PIAS1 at Ser90, which is required for its activity. In addition, PRMT1 induces arginine methylation of PIAS1 at Arg303 following interferon treatment and is associated with its repressive activity on Stat1. PIAS4, also known as PIASy, is a specific SUMO-E3 ligase for Ets-1 and represses Ets-1 dependent transcription. PIAS4 also alters the nuclear localization, reduces the transcriptional activity of C/EBPδ, and enhances cell proliferation and migration.,E3 SUMO-protein ligase PIAS4

Poids moléculaire: observerd band 56kDa

ID gène: 51588

UniProt: Q8N2W9

Pathways: Signalistation JAK/STAT, Interferon-gamma Pathway, Positive Regulation of Response to DNA Damage Stimulus