RFWD3 anticorps (AA 350-430)

N° du produit ABIN7223390

Cet anticorps Lapin Polyclonal détecte spécifiquement RFWD3 dans WB, ELISA, IHC et IF. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour RFWD3 anticorps (AA 350-430) (ABIN7223390)

Antigène: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFWD3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-RFWD3 anticorps

Épitope: AA 350-430

Fonction: Rabbit Anti-RFWD3 Polyclonal Antibody

Specificité: RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human RFWD3 at AA range: 350-430

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur RFWD3

Antigène: RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))

Autre désignation: RFWD3

Sujet: RFWD3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

Poids moléculaire: 85kD

ID gène: 55159

UniProt: Q6PCD5