SHB anticorps (pTyr246)

N° du produit ABIN7213118

Cet anticorps anti-SHB Polyclonal Lapin (ABIN7213118) détecte spécifiquement SHB dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour SHB anticorps (pTyr246) (ABIN7213118)

Antigène: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SHB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-SHB anticorps

Épitope: pTyr246

Fonction: Rabbit Anti-Shb (phospho Tyr246) Polyclonal Antibody

Specificité: Phospho-Shb (Y246) Polyclonal Antibody detects endogenous levels of Shb protein only when phosphorylated at Y246.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from human Shb Phospho-Tyr246

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur SHB

Antigène: SHB (Src Homology 2 Domain Containing Adaptor Protein B (SHB))

Autre désignation: Shb

Sujet: SHB, SH2 domain-containing adapter protein BSHB (SH2 homology protein in B/ beta -cells) is a 55-59 kDa cytoplasmic adaptor protein that serves as a link between phosphotyrosine residues and downstream signaling pathways. SHB is ubiquitously expressed, and binds tyrosine kinase receptors such as FGFR1, VEGFR2, and the TCR (zeta -chain) following their activation. It is highly modular, and through a variety of motifs, is able to bind multiple, structurally unrelated proteins that collectively generate a signal transduction network. Human SHB is 509 amino acids (aa) in length. It contains an N-terminal Pro-rich region that binds SH3 domain-containing proteins (aa 19-45), a central PTB domain that binds select aa motifs, and a C-terminal SH2 domain that interacts with phosphotyrosines (aa 410-504). There are at least nine utilized phosphorylation sites. There are two isoform variants. One is 66-68 kDa in size, and possesses an 87 aa Pro-rich N-terminal extension. This MW may increase to 77-80 kDa following posttranslational processing. A second variant shows an 18 aa substitution for aa 280-509. Over aa 2-128, human SHB shares 91 % aa identity with mouse SHB.

Poids moléculaire: 55kD

ID gène: 6461

UniProt: Q15464