UBE3B anticorps (AA 550-630)

N° du produit ABIN7225756

Cet anticorps anti-UBE3B Polyclonal Lapin (ABIN7225756) détecte spécifiquement UBE3B dans WB, ELISA, IHC et IF. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour UBE3B anticorps (AA 550-630) (ABIN7225756)

Antigène: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE3B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-UBE3B anticorps

Épitope: AA 550-630

Fonction: Rabbit Anti-UBE3B Polyclonal Antibody

Specificité: UBE3B Polyclonal Antibody detects endogenous levels of UBE3B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from the Internal region of human UBE3B at AA range: 550-630

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur UBE3B

Antigène: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Autre désignation: UBE3B

Sujet: UBE3B, Ubiquitin-protein ligase E3BThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: e1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. UBE3B (ubiquitin protein ligase E3B) encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for UBE3B.

Poids moléculaire: 123kD

ID gène: 89910

UniProt: Q7Z3V4