ZCCHC11 anticorps

N° du produit ABIN7231968

L’anticorps anti-ZCCHC11 Polyclonal Lapin est utilisé pour la détection de ZCCHC11 dans des échantillons de Humain et Souris. Il a été validé pour WB et ELISA.

Aperçu rapide pour ZCCHC11 anticorps (ABIN7231968)

Antigène: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ZCCHC11 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-ZCCHC11 anticorps

Fonction: Rabbit Anti-TUT4 Polyclonal Antibody

Specificité: The antibody detects endogenous levels of TUT4 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Synthesized peptide derived from part region of human TUT4 protein

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur ZCCHC11

Antigène: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Autre désignation: TUT4

Sujet: Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11ZCCHC11 is an RNA uridyltransferase (EC 2.7.7.52) that uses UTP to add uridines to the 3-prime end of substrate RNA molecules. GO annotations related to this gene include nucleic acid binding and nucleotidyltransferase activity. Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay. Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency. Does not bind RNA directly, but recruited to RNA targets by RNA-binding protein LIN28A. Also catalyzes the 3 uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult.

Poids moléculaire: 180kD

ID gène: 23318

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance