MLH1 anticorps

N° du produit ABIN7232932

Cet anticorps Souris Monoclonal détecte spécifiquement MLH1 dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour MLH1 anticorps (ABIN7232932)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MLH1 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: G409-1

Détail du produit anti-MLH1 anticorps

Fonction: Anti-MLH1 Mouse Monoclonal Antibody

Specificité: Human MLH1. Reactivity with other species has not been investigated.

Réactivité croisée: Humain

Immunogène: Recombinant human MLH1

Isotype: IgG2a

Information d'application

Indications d'application:
Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: Tris buffer, pH 7.3-7.7, 1 % BSA, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1

Sujet: DNA mismatch repair protein Mlh1,Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. {PubMed:16873062, PubMed:18206974, PubMed:20020535, PubMed:21120944, PubMed:9311737}.,Nucleus, Chromosome

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response