PINK1 anticorps (C-Term)

N° du produit ABIN7233046

Cet anticorps Souris Monoclonal détecte spécifiquement PINK1 dans WB, IHC et IF/ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour PINK1 anticorps (C-Term) (ABIN7233046)

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PINK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: S4-15

Détail du produit anti-PINK1 anticorps

Épitope: AA 112-496, C-Term

Fonction: Anti-PINK1 Mouse Monoclonal Antibody

Specificité: This antibody recognizes human, mouse, and rat PINK1.

Réactivité croisée: Humain, Souris, Rat

Immunogène: Fusion protein corresponding to aa 112-496 (cytoplasmic C-terminus) of human PINK1. This sequence is 82

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: use at 1-5 μg/mL. A band of ~50 kDa is detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: This product is stable for at least one (1) year at -20°C.

Détails sur PINK1

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Autre désignation: PINK1

Sujet: Serine/threonine-protein kinase PINK1, mitochondrial,PINK1 (PTEN-induced putative kinase 1) is a mitochondrial serine/threonine kinase that maintains mitochondrial function and integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy. PINK1 is synthesized as a 63 kDa protein which undergoes proteolytic processing to yield two cleaved forms, ~55 kDa and ~42 kDa. PINK1 may be localized to the outer mitochondrial membrane with its kinase domain facing the cytosol which would explain the observed physical interaction between PINK1 and cytosolic E3 ubiquitin ligase Parkin.

UniProt: Q9BXM7

Pathways: Autophagy