G Protein-Coupled Receptor 132 anticorps (Extracellular)

N° du produit ABIN7237639

L’anticorps Lapin Polyclonal anti-G Protein-Coupled Receptor 132 a été validé pour WB, FACS et LCI. Il convient pour détecter G Protein-Coupled Receptor 132 dans des échantillons de Souris.

Aperçu rapide pour G Protein-Coupled Receptor 132 anticorps (Extracellular) (ABIN7237639)

Antigène: G Protein-Coupled Receptor 132 (GPR132)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp G Protein-Coupled Receptor 132 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-G Protein-Coupled Receptor 132 anticorps

Épitope: AA 184-198, Extracellular

Fonction: A Rabbit Polyclonal antibody to GPR132 (extracellular)

Specificité: Extracellular, 2nd loop.

Réactivité croisée: Souris, Rat

Homologie: Rat - 11 out of 15 amino acid residues identical It won't recognize human GPR132

Attributs du produit: Extracellular, 2nd loop

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: GST fusion protein,

Immunogen Sequence: Peptide CFEPLRMNSKIAGYH, corresponding to amino acid residues 184 - 198 of mouse GPR132

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235365)

Blocking Peptide: (ABIN7235365)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur G Protein-Coupled Receptor 132

Antigène: G Protein-Coupled Receptor 132 (GPR132)

Autre désignation: GPR132 

Sujet: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A,G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

Alternative names: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A

ID gène: 56696

NCBI Accession: NM_013345

UniProt: Q9Z282