HCAR2 anticorps (Extracellular)

N° du produit ABIN7237697

Cet anticorps anti-HCAR2 est un anticorps Lapin Polyclonal détectant HCAR2 dans IHC, WB, FACS et LCI. Adapté pour Souris.

Aperçu rapide pour HCAR2 anticorps (Extracellular) (ABIN7237697)

Antigène: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HCAR2 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-HCAR2 anticorps

Épitope: AA 82-94, Extracellular

Fonction: A Rabbit Polyclonal antibody to GPR109A/HCAR2 (extracellular)

Specificité: Extracellular, 1st loop.

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - 11 out of 13 amino acid residues identical, Human - 9 out of 13 amino acid residues identical Note: it is not expected to recognize the highly homologous human protein HCAR3 (Accession P49019) HCAR3 has no mouse,rat orthologs

Attributs du produit: Extracellular, 1st loop.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DNYVHNWDWRFGG, corresponding to amino acid residues 82 - 94 of mouse HCAR2

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235778)

Blocking Peptide: (ABIN7235778)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur HCAR2

Antigène: HCAR2 (Hydroxycarboxylic Acid Receptor 2 (HCAR2))

Autre désignation: HCAR2

Sujet: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG,Hydroxy-carboxylic acid receptors (HCA1-3) are a family of G-protein-coupled receptors that regulate lipolysis. HCAR2 (GPR109A) was identified as the receptor for nicotinic acid (niacin, vitamin B3) and beta-hyroxybutyrate (β-OHB), a ketone body synthesized by the liver under negative energy balance1.HCAR2 is mainly expressed on adipocytes and immune cells and in lower levels in retinal pigmented and colonic epithelial cells, microglia and normal mammary tissue. High levels of HCAR2 can be found in immune cells such as macrophages in response to the presence of interferon-γ, TNF-α and hypoxia2.HCAR2 activity in adipocytes promotes inhibition of adenylate cyclase and decrease cAMP levels. HCAR2 activity leads to a rapid decrease in lipolysis and reduction in the release of fatty acids from the adipocytes3.Activation of HCAR2 has also been associated with anti-inflammatory effects in different disease states such as sepsis, diabetes and obesity. Interaction of HCAR2 with niacin and β-OHB under this conditions lead to variety of signaling events involving inflammatory downstream targets4.

Alternative names: Hydroxycarboxylic Acid Receptor 2, Niacin Receptor 1, G-Protein Coupled Receptor HM74A, HM74A, Nicotinic acid receptor, PUMAG

ID gène: 80885

NCBI Accession: NM_177551

UniProt: Q9EP66