Recombinant DLG4 anticorps (Alexa Fluor 647)

N° du produit ABIN7272870

L’anticorps anti-DLG4 Monoclonal Alpaca est utilisé pour la détection de DLG4 dans des échantillons de Rat et Souris. Il a été validé pour WB, IHC, ICC et IF.

Aperçu rapide pour Recombinant DLG4 anticorps (Alexa Fluor 647) (ABIN7272870)

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Rat, Souris

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp DLG4 est conjugé à/à la Alexa Fluor 647

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1B2

Détail du produit anti-DLG4 anticorps (Alexa Fluor 647)

Expression System: E.coli

Specificité: Recognizes mouse and rat PSD95. Other species not tested

Purification: Produced in: E.coli

Immunogène: anti-PSD95 is a fluorescently conjugated single-domain antibody that recognizes mouse and rat PSD95.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: IF: 1:500 (corresponding to 5 nM final concentration) , 5 µg/mL (IHC),

Commentaires:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Détails sur DLG4

Antigène: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Autre désignation: PSD95

Sujet: PSD95 is a member of the MAGUK family, and it plays an important role in the formation of a dynamic scaffold at the post synapses. It functions as an organizing centrum of receptors, ion channels, kinases, and other relevant players in achieving synaptic plasticity and molecular changes during long-term potentiation.

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity