Recombinant SLC17A7 anticorps (Atto 488)

N° du produit ABIN7272877

Cet anticorps anti-SLC17A7 Monoclonal Alpaca conjugué à Atto 488 (Clone Nb9) (ABIN7272877) détecte spécifiquement SLC17A7 dans ICC, IF et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant SLC17A7 anticorps (Atto 488) (ABIN7272877)

Antigène: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivité: Humain

Hôte: Alpaca

Clonalité: Monoclonal

Conjugué: Cet anticorp SLC17A7 est conjugé à/à la Atto 488

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Nb9

Détail du produit anti-SLC17A7 anticorps (Atto 488)

Expression System: E.coli

Specificité: Human VGlut1, other species not yet tested, no significant cross-reaction with VGlut2 or VGlut9

Purification: Produced in: E.coli

Immunogène: anti-VGLUT1 is derived from a single-domain antibody (sdAb) recognizing specifically VGLUT1 with high affinity and specificity.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Détails sur SLC17A7

Antigène: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Autre désignation: VGlut1

Sujet: The vesicula glutamate transport 1 (vGlut1) is primarily located on the synaptic vesicles of glutamatergic synapses (excitatory). Its function is to fill the vesicles with the neurotransmitter glutamate before they fuse to the plasma membrane and release their content from the pre-synaptic compartment into the synaptic cleft to stimulate postsynaptic receptors. vGlut1 uses the energy from the proton gradient generated by the vesicular ATPase to transport glutamate inside the vesicle.