Recombinant RPA2 anticorps (pSer4, pSer8)

N° du produit ABIN7453379

L’anticorps Lapin Monoclonal anti-RPA2 a été validé pour WB, IP, ICC et IF/ICC. Il convient pour détecter RPA2 dans des échantillons de Humain.

Aperçu rapide pour Recombinant RPA2 anticorps (pSer4, pSer8) (ABIN7453379)

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp RPA2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (fixed cells) (IF/ICC)

Clone: BL-165-5F1

Détail du produit anti-RPA2 anticorps

Épitope: pSer4, pSer8

Fonction: Rabbit anti-Phospho RPA32 (S4/S8) Recombinant Monoclonal Antibody [BL-165-5F1]

Immunogène: surrounding serine 4/8

Isotype: IgG

Information d'application

Indications d'application:

ICC-IF: 1:1,000 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IP: 6 μL/1.0 mg lysate

ICC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

WB: 17:40:00

Restrictions: For Research Use only

Stockage

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détails sur RPA2

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Autre désignation: RPA32

Sujet: Background: Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32 kDa (RPA2), and 14 kDa (RPA3). RPA2, the 32 kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.

ID gène: 6118

NCBI Accession: NP_002937

UniProt: P15927

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA