beta Actin anticorps (C-Term)

N° du produit ABIN7464220

L’anticorps Lapin Polyclonal anti-beta Actin a été validé pour WB, IF, IHC (p), ICC, IP et IHC (fro). Il convient pour détecter beta Actin dans des échantillons de Humain.

Aperçu rapide pour beta Actin anticorps (C-Term) (ABIN7464220)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Classe de qualité: KO Validated

Détail du produit anti-beta Actin anticorps

Épitope: C-Term

Specificité: IP/MS validation was supported by references (PMID:30377395)

Réactivité croisée: C. elegans, Candida albicans, Cat, Poulet, Chien, Drosophila melanogaster, E. coli, Hamster, Cheval, Humain, Singe, Souris, Onchocerca, Oryza sativa, Porc, Plantes, Rat, Saccharomyces cerevisiae, Mouton, Tree shrew, Xenopus laevis

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T , A431 , Jurkat , Raji , mouse kidney , mouse lung , mouse testis , Candida albicans (sc5314) , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , Rat-2 , NTH-3T3 , mouse brain , PC-12 , Rat brain

Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.4 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: actin beta

Sujet: Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Poids moléculaire: 42 kDa

ID gène: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction