AFT1 anticorps (pSer63)

N° du produit ABIN7464253

L’anticorps Lapin Polyclonal anti-AFT1 a été validé pour WB. Il convient pour détecter AFT1 dans des échantillons de Humain.

Aperçu rapide pour AFT1 anticorps (pSer63) (ABIN7464253)

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AFT1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-AFT1 anticorps

Épitope: pSer63

Specificité: This antibody is raised against human ATF1 phosphorylated at Ser63, and it also may detect the phosphorylated CREB protein due to sequence homology.

Réactivité croisée: Humain, Souris

Purification: Purified by antigen-affinity chromatography.

Immunogène: Carrier-protein conjugated synthetic peptide surrounding phospho Ser63 of human ATF1. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 293T (25 μg/ml anisomycin treatment for 30 min) , A431(200 ng/ml EGF treatment for 30 min) , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.94 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur AFT1

Antigène: AFT1 (Activating Transcription Factor 1 (AFT1))

Autre désignation: activating transcription factor 1

Sujet: Activating transcription factor 1 , EWS-ATF1 , FUS/ATF-1 , TREB36,This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq]

Poids moléculaire: 29 kDa

ID gène: 466

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades