beta Actin anticorps (C-Term)

N° du produit ABIN7464288

Cet anticorps Souris Monoclonal détecte spécifiquement beta Actin dans WB, IF, IHC (p), ICC et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour beta Actin anticorps (C-Term) (ABIN7464288)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Classe de qualité: KO Validated

Clone: GT5512

Détail du produit anti-beta Actin anticorps

Épitope: C-Term

Specificité: IP/MS validation was supported by references (PMID:30377426)

Réactivité croisée: Cat, Chien, Drosophila melanogaster, Chévre, Humain, Singe, Souris, Lapin, Rat, Saccharomyces cerevisiae, Xenopus laevis, Poisson zèbre (Danio rerio)

Purification: Affinity purified by Protein G.

Immunogène: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: 30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

Validation: IP/MS, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: actin beta

Sujet: Actin beta , BRWS1 , PS1TP5BP1,This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Poids moléculaire: 42 kDa

ID gène: 60

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction