Cyclin B1 anticorps

N° du produit ABIN7464490

L’anticorps Souris Monoclonal anti-Cyclin B1 a été validé pour WB et IHC (p). Il convient pour détecter Cyclin B1 dans des échantillons de Humain.

Aperçu rapide pour Cyclin B1 anticorps (ABIN7464490)

Antigène: Cyclin B1 (CCNB1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin B1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GT238

Détail du produit anti-Cyclin B1 anticorps

Réactivité croisée: Humain

Purification: Affinity purified by Protein G.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HeLa (2mM thymidine treatment and recovery for 3.5hr) , HeLa (2mM thymidine treatment and recovery for 12hr) , U2OS (100ng/ml Nocodazole treatment for 24hr)

Validation: Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur Cyclin B1

Antigène: Cyclin B1 (CCNB1)

Autre désignation: cyclin B1

Sujet: Cyclin B1 , CCNB,The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]

Poids moléculaire: 48 kDa

ID gène: 891

UniProt: P14635

Pathways: Cycle Cellulaire, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase