MCM4 anticorps (C-Term)

N° du produit ABIN7464669

Cet anticorps Lapin Polyclonal détecte spécifiquement MCM4 dans WB, IHC (p), IF, IP, ICC et ChIP. Il présente une réactivité envers Humain.

Aperçu rapide pour MCM4 anticorps (C-Term) (ABIN7464669)

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-MCM4 anticorps

Épitope: C-Term

Specificité: IP/MS validation was supported by references (PMID:30377397)

Réactivité croisée: Humain, Souris, Poisson zèbre (Danio rerio)

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Jurkat , Raji , NCI-H929 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Validation: IP/MS

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur MCM4

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Autre désignation: minichromosome maintenance complex component 4

Sujet: Minichromosome maintenance complex component 4 , CDC21 , CDC54 , IMD54 , NKCD , NKGCD , P1-CDC21 , hCdc21,The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]

Poids moléculaire: 97 kDa

ID gène: 4173

UniProt: P33991

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA