CHFR anticorps (N-Term)

N° du produit ABIN7467770

Cet anticorps anti-CHFR est un anticorps Lapin Polyclonal détectant CHFR dans ICC et IF. Adapté pour Humain.

Aperçu rapide pour CHFR anticorps (N-Term) (ABIN7467770)

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHFR est non-conjugé

Application: Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-CHFR anticorps

Épitope: N-Term

Réactivité croisée: Humain

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human CHFR. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur CHFR

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Autre désignation: checkpoint with forkhead and ring finger domains

Sujet: Checkpoint with forkhead and ring finger domains , RNF116 , RNF196,E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Poids moléculaire: 73 kDa

ID gène: 55743

UniProt: Q96EP1