Double-Strand Break Repair Protein MRE11 (MRE11) anticorps

N° du produit ABIN7468276

L’anticorps anti-Double-Strand Break Repair Protein MRE11 Polyclonal Lapin est utilisé pour la détection de Double-Strand Break Repair Protein MRE11 dans des échantillons de Humain. Il a été validé pour ICC, IF, IP, WB et IHC (p).

Aperçu rapide pour Double-Strand Break Repair Protein MRE11 (MRE11) anticorps (ABIN7468276)

Antigène: Double-Strand Break Repair Protein MRE11 (MRE11)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human Mre11. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: A431 , C8D30 , NIH-3T3 , PC-12 , HeLa , MRE11A-transfected 293T Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.9 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: Double-Strand Break Repair Protein MRE11 (MRE11)

Autre désignation: MRE11 homolog, double strand break repair nuclease

Sujet:

Synonyms: MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Background: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Poids moléculaire: 81 kDa

ID gène: 4361

UniProt: P49959