Double-Strand Break Repair Protein MRE11 (MRE11) (AA 182-582) anticorps

N° du produit ABIN7468277

Cet anticorps anti-Double-Strand Break Repair Protein MRE11 Monoclonal Souris (Clone 12D7) (ABIN7468277) détecte spécifiquement Double-Strand Break Repair Protein MRE11 dans ICC, IF, IP, WB, IHC (p), ELISA, Func et PLA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Double-Strand Break Repair Protein MRE11 (MRE11) (AA 182-582) anticorps (ABIN7468277)

Antigène: Double-Strand Break Repair Protein MRE11 (MRE11)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Functional Studies (Func), Proximity Ligation Assay (PLA)

Classe de qualité: KO Validated

Clone: 12D7

Détail du produit

Épitope: AA 182-582

Réactivité croisée: Humain, Rat

Purification: Protein G purified

Immunogène: Amino acids 182-582 of Mre11 expressed in E. coli.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HeLa , Jurkat , Raji , NCI-H929 , 293T , human Mre11-transfected 293T , PC-12 Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détail du antigène

Antigène: Double-Strand Break Repair Protein MRE11 (MRE11)

Autre désignation: MRE11 homolog, double strand break repair nuclease

Sujet:

Synonyms: MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Background: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Poids moléculaire: 81 kDa

ID gène: 4361

UniProt: P49959