ERO1L anticorps

N° du produit ABIN7468316

Cet anticorps anti-ERO1L est un anticorps Lapin Polyclonal détectant ERO1L dans WB, IHC (p), ICC et IF. Adapté pour Humain.

Aperçu rapide pour ERO1L anticorps (ABIN7468316)

Antigène: ERO1L (ERO1-Like Protein alpha (ERO1L))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERO1L est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-ERO1L anticorps

Réactivité croisée: Poulet, Humain, Souris

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the center region of human ERO1L. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: mouse stomach , HCT116 , MCF-7

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.95 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ERO1L

Antigène: ERO1L (ERO1-Like Protein alpha (ERO1L))

Autre désignation: endoplasmic reticulum oxidoreductase 1 alpha

Sujet:

Synonyms: endoplasmic reticulum oxidoreductase 1 alpha , ERO1-L , ERO1-L-alpha , ERO1-alpha , ERO1L , ERO1LA , Ero1alpha

Background: Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.

Poids moléculaire: 54 kDa

ID gène: 30001

UniProt: Q96HE7

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Brown Fat Cell Differentiation