RAD50 anticorps (AA 1-425)

N° du produit ABIN7468849

L’anticorps Souris Monoclonal anti-RAD50 a été validé pour WB, IHC, IP, ICC, IF, IHC (p) et in vitro. Il convient pour détecter RAD50 dans des échantillons de Humain.

Aperçu rapide pour RAD50 anticorps (AA 1-425) (ABIN7468849)

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAD50 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), In vitro Assay (in vitro)

Classe de qualité: KO Validated

Clone: 13B3

Détail du produit anti-RAD50 anticorps

Épitope: AA 1-425

Réactivité croisée: Humain, Singe, Souris, Rat

Purification: Affinity purified by Protein G.

Immunogène: Amino acids 1-425 of Rad50 expressed in E. coli.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: HeLa , HeLa nuclear extract Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.24 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur RAD50

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Autre désignation: RAD50 double strand break repair protein

Sujet:

Synonyms: RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]

Poids moléculaire: 154 kDa

ID gène: 10111

UniProt: Q92878

Pathways: Réparation de l'ADN, Protein targeting to Nucleus