RAD50 anticorps (N-Term)

N° du produit ABIN7468850

Cet anticorps anti-RAD50 est un anticorps Lapin Polyclonal détectant RAD50 dans WB, IP et IHC (p). Adapté pour Humain.

Aperçu rapide pour RAD50 anticorps (N-Term) (ABIN7468850)

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD50 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Classe de qualité: KO Validated

Détail du produit anti-RAD50 anticorps

Épitope: N-Term

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human Rad50. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: Neuro2A , GL261 , NT2D1 , HeLa nuclear extract , PC-12 , HeLa Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.78 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur RAD50

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Autre désignation: RAD50 double strand break repair protein

Sujet:

Synonyms: RAD50 double strand break repair protein , NBSLD , RAD502 , hRad50

Background: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq]

Poids moléculaire: 154 kDa

ID gène: 10111

UniProt: Q92878

Pathways: Réparation de l'ADN, Protein targeting to Nucleus