Recombinant Caldesmon anticorps
Aperçu rapide pour Recombinant Caldesmon anticorps (ABIN7477385)
Antigène
Voir toutes Caldesmon (CALD1) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Immunogène
- Recombinant human full-length CALD1 protein
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Colon: A strong caldesmon staining should be seen of the muscularis mucosa and of a membrane-like layer along the basement membranes of crypts (intestinal subepithelial myofibroblasts).
Negative Control: Colon: Normal epithelial cells must not show caldesmon staining.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Caldesmon (CALD1) (Caldesmon 1 (CALD1))
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Autre désignation
- Caldesmon
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Sujet
- CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22,Caldesmon antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- Q05682
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Pathways
- Myometrial Relaxation and Contraction
Antigène
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