CD31 anticorps (AA 625-738)
Aperçu rapide pour CD31 anticorps (AA 625-738) (ABIN7477409)
Antigène
Voir toutes CD31 (PECAM1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 625-738
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Immunogène
- Recombinant fragment (around aa 625-738) of human CD31 protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Liver: At least a weak to moderate staining of the majority of the hepatic sinusoidal endothelial cells should be seen.
Negative Control: Liver: Hepatocytes should not show any CD31 staining.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Autre désignation
- CD31
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Sujet
- EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA,CD31 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P16284
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Pathways
- Regulation of Actin Filament Polymerization
Antigène
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