Recombinant CD34 anticorps

N° du produit ABIN7477411

Cet anticorps anti-CD34 est un anticorps Lapin Monoclonal détectant CD34 dans IHC. Adapté pour Humain.

Aperçu rapide pour Recombinant CD34 anticorps (ABIN7477411)

Antigène: CD34

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-034R

Détail du produit anti-CD34 anticorps

Immunogène: Recombinant full-length human HPCA1 protein

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level.

Negative Control: Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P28906