Recombinant Elastase 3B anticorps (AA 82-238)
Aperçu rapide pour Recombinant Elastase 3B anticorps (AA 82-238) (ABIN7477421)
Antigène
Voir toutes Elastase 3B (ELA3B) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Épitope
- AA 82-238
-
Immunogène
- Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
-
Isotype
- IgG
-
-
-
-
Indications d'application
- IHC 1:100-1:200
-
Commentaires
-
Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance.
Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells.
-
Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Stock
- 4 °C,-20 °C,-80 °C
-
Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
-
Autre désignation
- CELA3B
-
Sujet
- Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E,CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P08861
Antigène
-