Recombinant Chromogranin A anticorps

N° du produit ABIN7477423

Cet anticorps anti-Chromogranin A est un anticorps Lapin Monoclonal détectant Chromogranin A dans IHC. Adapté pour Humain.

Aperçu rapide pour Recombinant Chromogranin A anticorps (ABIN7477423)

Antigène: Chromogranin A (CHGA)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Chromogranin A est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-380R

Détail du produit anti-Chromogranin A anticorps

Immunogène: Recombinant full-length human chromogranin A protein

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Appendix: An at least weak to moderate staining must be seen in the axons and ganglion cells of the peripheral nerves while mucosal neuroendocrine cells exhibit a strong staining (neuroendocrine cells are not sufficient as positive controls because they express CGA so strongly, that even inefficient staining will detect its expression)

Negative Control: Appendix: Epithelial and smooth muscle cells should be negative.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Chromogranin A

Antigène: Chromogranin A (CHGA)

Autre désignation: Chromogranin A

Sujet: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling