Recombinant Collagen IV anticorps
Aperçu rapide pour Recombinant Collagen IV anticorps (ABIN7477425)
Antigène
Voir toutes Collagen IV (COL4) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- Collagen IV antibody (704R)
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Immunogène
- Purified human placental extract
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.
Product Description/Background: Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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Date de péremption
- 24 months
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- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Autre désignation
- Collagen IV
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Sujet
- Gene Alias: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR
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UniProt
- P02462, P08572, P29400, P53420
Antigène
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