Recombinant Collagen IV anticorps

N° du produit ABIN7477425

Cet anticorps Lapin Monoclonal détecte spécifiquement Collagen IV dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Collagen IV anticorps (ABIN7477425)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-704R

Détail du produit anti-Collagen IV anticorps

Immunogène: Purified human placental extract

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen.

Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: Collagen IV

Sujet: Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, COL4A3, COL4A4, COL4A5, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, Collagen Type IV Alpha 3, Collagen Type IV Alpha 4, Collagen Type IV Alpha 5, RATOR,Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02462, P08572, P29400, P53420