Recombinant Elastin anticorps
Aperçu rapide pour Recombinant Elastin anticorps (ABIN7477467)
Antigène
Voir toutes Elastin (ELN) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Immunogène
- Recombinant full-length human Elastin protein
-
Isotype
- IgG
-
-
-
-
Indications d'application
- IHC 1:100-1:200
-
Commentaires
-
Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).
Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells.
-
Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Stock
- 4 °C,-20 °C,-80 °C
-
Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Elastin (ELN)
-
Autre désignation
- Elastin
-
Sujet
- Elastin, ELN, SVAS, Tropoelastin, WBS, WS,Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P15502
-
Pathways
- Regulation of Actin Filament Polymerization
Antigène
-