Recombinant MBP anticorps (AA 150-250)
Aperçu rapide pour Recombinant MBP anticorps (AA 150-250) (ABIN7477511)
Antigène
Voir toutes MBP AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 150-250
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Immunogène
- Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Brain: A fibrillar staining pattern should be seen.
Negative Control: Colon: MBP staining should be completely absent.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MBP (Myelin Basic Protein (MBP))
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Autre désignation
- Myelin Basic Protein
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Sujet
- GDB, Golli MBP, myelin basic protein, Hemopoietic MBP, HMBPR, HUGO, MLD, Myelin A1 Protein, basic, Myelin Deficient, Myelin membrane encephalitogenic protein, SHI, Shiverer, SP,Myelin Basic Protein antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P02686
Antigène
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