Recombinant MCM7 anticorps (AA 195-319)
Aperçu rapide pour Recombinant MCM7 anticorps (AA 195-319) (ABIN7477515)
Antigène
Voir toutes MCM7 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 195-319
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Immunogène
- Recombinant human MCM7 protein fragment (aa195-319)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.
Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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Autre désignation
- MCM7
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Sujet
- CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Minichromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P33993
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Pathways
- Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
Antigène
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