Recombinant MSH6 anticorps (AA 1-100)

N° du produit ABIN7477533

Cet anticorps Lapin Monoclonal détecte spécifiquement MSH6 dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant MSH6 anticorps (AA 1-100) (ABIN7477533)

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-906R

Détail du produit anti-MSH6 anticorps

Épitope: AA 1-100

Immunogène: Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:75-1:150

Commentaires:

Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

Negative Control: Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6

Sujet: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein,MSH6 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P52701

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response