ENO2/NSE anticorps (AA 416-433)

N° du produit ABIN7477539

Cet anticorps Souris Monoclonal détecte spécifiquement ENO2/NSE dans IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour ENO2/NSE anticorps (AA 416-433) (ABIN7477539)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-451M

Détail du produit anti-ENO2/NSE anticorps

Épitope: AA 416-433

Immunogène: A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.

Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: NSE gamma

Sujet: ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE , -phospho-D-glycerate hydrolyase,NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09104