Recombinant Nucleolin anticorps (full length)
Aperçu rapide pour Recombinant Nucleolin anticorps (full length) (ABIN7470651)
Antigène
Voir toutes Nucleolin (NCL) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- full length
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Fonction
- Nucleolin antibody (623R)
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Immunogène
- Recombinant full-length human NCL protein
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.
Product Description: Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
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Protocole
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Date de péremption
- 24 months
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- Nucleolin (NCL)
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Autre désignation
- Nucleolin
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Sujet
- Gene Alias: NCL, Nucl, Nucleolin, Protein C25
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UniProt
- P19338
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Pathways
- Ribonucleoprotein Complex Subunit Organization
Antigène
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