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Occludin anticorps (AA 282-415)

Cet anticorps anti-Occludin est un anticorps Souris Monoclonal détectant Occludin dans IHC. Adapté pour Humain.
N° du produit ABIN7477543

Aperçu rapide pour Occludin anticorps (AA 282-415) (ABIN7477543)

Antigène

Voir toutes Occludin (OCLN) Anticorps
Occludin (OCLN)

Reactivité

  • 113
  • 63
  • 45
  • 34
  • 18
  • 13
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
Humain

Hôte

  • 91
  • 28
  • 3
  • 1
Souris

Clonalité

  • 86
  • 37
Monoclonal

Conjugué

  • 56
  • 10
  • 6
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Occludin est non-conjugé

Application

  • 102
  • 35
  • 31
  • 30
  • 26
  • 26
  • 17
  • 16
  • 13
  • 12
  • 10
  • 6
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC)

Clone

MSVA-415M
  • Épitope

    • 16
    • 16
    • 12
    • 11
    • 9
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 282-415

    Immunogène

    ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

    Isotype

    IgG
  • Indications d'application

    IHC 1:100-1:200

    Commentaires

    Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

    Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

    Protocole

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Stock

    4 °C,-20 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Antigène

    Occludin (OCLN)

    Autre désignation

    Occludin

    Sujet

    BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    Q16625

    Pathways

    Cell-Cell Junction Organization, Hepatitis C
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