Recombinant Prostate Specific Antigen anticorps (AA 150-250)
Aperçu rapide pour Recombinant Prostate Specific Antigen anticorps (AA 150-250) (ABIN7477569)
Antigène
Voir toutes Prostate Specific Antigen (PSA) AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 150-250
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Immunogène
- Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction.
Negative Control: Colon: PSA immunostaining should be absent in all cells.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Prostate Specific Antigen (PSA)
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Autre désignation
- PSA
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Sujet
- Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin,PSA antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P07288
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Pathways
- Système du Complément
Antigène
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