Recombinant Synaptophysin anticorps (AA 274-313)

N° du produit ABIN7477577

L’anticorps Lapin Monoclonal anti-Synaptophysin a été validé pour IHC. Il convient pour détecter Synaptophysin dans des échantillons de Humain.

Aperçu rapide pour Recombinant Synaptophysin anticorps (AA 274-313) (ABIN7477577)

Antigène: Synaptophysin (SYP)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Synaptophysin est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-462R

Détail du produit anti-Synaptophysin anticorps

Épitope: AA 274-313

Immunogène: Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Colon: A moderate to strong staining of the axons of the Auerbach's and Meissner's plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.

Negative Control: Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Synaptophysin

Antigène: Synaptophysin (SYP)

Autre désignation: Synaptophysin

Sujet: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH,Synaptophysin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity