Recombinant S100A1 anticorps
Aperçu rapide pour Recombinant S100A1 anticorps (ABIN7477581)
Antigène
Voir toutes S100A1 AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Immunogène
- Recombinant full-length human S100B protein
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Isotype
- IgG
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Indications d'application
- IHC 1:100-1:200
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Commentaires
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Positive Control: Appendix: Schwann cells of peripheral nerves and adipocytes should show a strong, predominantly cytoplasmic S100B staining.
Negative Control: Appendix: Smooth muscle and epithelial cells must not show any S100B staining.
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Protocole
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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Autre désignation
- S100
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Sujet
- NEF, Protein S100-B, S-100 protein beta chain, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100beta,S100 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P04271
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Pathways
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
Antigène
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