Uroplakin 1B anticorps (AA 109-229)

N° du produit ABIN7477613

L’anticorps Souris Monoclonal anti-Uroplakin 1B a été validé pour IHC. Il convient pour détecter Uroplakin 1B dans des échantillons de Humain.

Aperçu rapide pour Uroplakin 1B anticorps (AA 109-229) (ABIN7477613)

Antigène: Uroplakin 1B (UPK1B)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Uroplakin 1B est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-734M

Détail du produit anti-Uroplakin 1B anticorps

Épitope: AA 109-229

Immunogène: Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).

Negative Control: Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Uroplakin 1B

Antigène: Uroplakin 1B (UPK1B)

Autre désignation: Uroplakin 1B

Sujet: Tetraspanin-20, Tspan-20, TSPAN20, UP1b, UPIb, UPK1B, Uroplakin-1b,Uroplakin 1B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O75841