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E. Coli anticorps (FITC)

Cet anticorps anti-E. Coli est un anticorps Lapin Polyclonal détectant E. Coli dans Func. Adapté pour E. coli.
N° du produit ABIN7478173

Aperçu rapide pour E. Coli anticorps (FITC) (ABIN7478173)

Antigène

E. Coli

Reactivité

  • 49
  • 11
E. coli

Hôte

  • 22
  • 21
  • 16
  • 1
Lapin

Clonalité

  • 34
  • 21
Polyclonal

Conjugué

  • 29
  • 10
  • 9
  • 9
  • 2
  • 1
Cet anticorp E. Coli est conjugé à/à la FITC

Application

  • 23
  • 22
  • 22
  • 18
  • 7
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Functional Studies (Func)
  • Specificité

    All "O" and "K" antigenic serotypes of Escherichia coli

    Réactivité croisée (Details)

    Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

    Purification

    This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Immunogène

    Mixtures of all antigenic serotypes

    Isotype

    IgG
  • Indications d'application

    TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
    Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). Will remove E. coli proteins from recombinant preparations

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
  • Antigène

    E. Coli

    Classe de substances

    Bacteria
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