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E. Coli anticorps (Peroxidase (POD))

Reactivité: E. coli WB, EIA, IHC Hôte: Chèvre Polyclonal Peroxidase (POD)
N° du produit ABIN7478178
  • Antigène Tous les produits E. Coli
    E. Coli
    Reactivité
    • 46
    • 11
    E. coli
    Hôte
    • 21
    • 18
    • 17
    • 1
    Chèvre
    Clonalité
    • 34
    • 18
    Polyclonal
    Conjugué
    • 27
    • 11
    • 9
    • 8
    • 1
    • 1
    Cet anticorp E. Coli est conjugé à/à la Peroxidase (POD)
    Application
    • 22
    • 21
    • 20
    • 17
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
    Specificité
    Many "O" and "K" antigenic serotypes of Escherichia coli
    Réactivité croisée (Details)
    Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
    Purification
    This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
    Immunogène
    Mixture of E. coli serotypes
    Isotype
    IgG
  • Indications d'application
    TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
    This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
    Agent conservateur
    Thimerosal (Merthiolate)
    Précaution d'utilisation
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
  • Antigène
    E. Coli
    Abstract
    E. Coli Produits
    Classe de substances
    Bacteria
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