Cet anticorps anti-E. Coli Polyclonal Lapin conjugué à FITC (ABIN7478173) détecte spécifiquement E. Coli dans Func.
L’anticorps est réactif avec des échantillons de E. coli.
Aperçu rapide pour E. Coli anticorps (FITC) (ABIN7478173)
Antigène
E. Coli
Reactivité
E. coli
Hôte
Lapin
Clonalité
Polyclonal
Conjugué
Cet anticorp E. Coli est conjugé à/à la FITC
Application
Functional Studies (Func)
Specificité
All "O" and "K" antigenic serotypes of Escherichia coli
Réactivité croisée (Details)
Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
Purification
This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
Reactivité: E. coli
ELISA, WB, IHC, FM
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12 Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). Will remove E. coli proteins from recombinant preparations
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.