E. Coli anticorps (Peroxidase (POD))
Aperçu rapide pour E. Coli anticorps (Peroxidase (POD)) (ABIN7478178)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Many "O" and "K" antigenic serotypes of Escherichia coli
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Réactivité croisée (Details)
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
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Purification
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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Immunogène
- Mixture of E. coli serotypes
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Isotype
- IgG
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Indications d'application
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TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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Agent conservateur
- Thimerosal (Merthiolate)
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Précaution d'utilisation
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- E. Coli
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Classe de substances
- Bacteria
Antigène
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