Recombinant Fc gamma RII (CD32) anticorps

N° du produit ABIN7487694

L’anticorps Lapin Chimeric anti- a été validé pour ELISA, IF, IP, BR et InhA. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour Recombinant Fc gamma RII (CD32) anticorps (ABIN7487694)

Antigène: Fc gamma RII (CD32)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Inconjugué

Application: ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: IV-3

Détail du produit

Fonction: Anti-CD32 [IV.3], Rabbit IgG, kappa

Specificité: This antibody recognizes and binds CD32a.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: The original antibody was generated against the FcR of K562 cells.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic ""FCGR2A"" mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318