alpha Tubulin anticorps

N° du produit ABIN7505839

L’anticorps Rat Monoclonal anti-alpha Tubulin a été validé pour WB, ELISA, IHC (p), IHC (fro) et ICFC. Il convient pour détecter alpha Tubulin dans des échantillons de Humain, Souris, Rat, Saccharomyces cerevisiae, Mammifères et Avian.

Aperçu rapide pour alpha Tubulin anticorps (ABIN7505839)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Souris, Rat, Saccharomyces cerevisiae, Mammifères, Avian

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: YOL1-34

Détail du produit anti-alpha Tubulin anticorps

Fonction: Anti-alpha-Tubulin Purified

Specificité: The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.

Purification: Purified by protein-G affinity chromatography.

Immunogène: Yeast tubulin

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry: Recommended dilution 4-8 μg/mL.
Western blotting: Recommended dilution 1-2 μg/mL, reducing conditions.
Flow cytometry: Recommended dilution: 8-12 μg/mL, intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-Tubulin

Sujet: Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA

ID gène: 7277

UniProt: Q71U36

Pathways: Dynamique des Microtubules